Food and Agricultural Immunology | |
A flow-injection chemiluminescent biomimetic immunoassay method using a molecularly imprinted polymer as a biomimetic antibody for the determination of trichlorfon | |
Zeliang Ju1  Shoumin Wang2  Zhixiang Xu2  Xuguang Qiao2  Mingdi Jiang2  | |
[1] Agricultural Bureau of Linqu, Linqu, People’s Republic of Chin;Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Taian, People’s Republic of Chin; | |
关键词: Flow injection; chemiluminescence; molecularly imprinted polymer; biomimetic immunoassay; | |
DOI : 10.1080/09540105.2017.1364707 | |
来源: publisher | |
【 摘 要 】
In this study, a molecularly imprinted polymer (MIP) was synthesized using trichlorfon as a template molecule. The MIP exhibited a high adsorption capacity toward trichlorfon. Using bovine serum albumin as a carrier, trichlorfon hapten was indirectly labeled with luminol, which competed with free trichlorfon for the combination with a biomimetic antibody of MIP. Based on this direct competitive format, a high throughput flow-injection chemiluminescent biomimetic immunoassay method was developed for fast detection of trace trichlorfon. The influencing factors were investigated and optimized. Under the optimal condition, a lower limit of detection (IC15) of 0.0024 mg/L was obtained using this method. This method was also used to detect the trichlorfon spiked in carrot and cabbage samples, and the results were also verified using the method of gas chromatography.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO202004239675610ZK.pdf | 1277KB | download |