Development of molecularly imprinted biomimetic immunoassay method based on quantum dot marker for detection of phthalates

Food and Agricultural Immunology

Ximo Wang¹  Zhixiang Xu¹  Longhua Xu¹  Chen Chen¹  Hongyan Zhang² 

[1] Shandong Agricultural University;Shandong Normal University;

关键词: biomimetic immunoassay;    molecular imprinting;    quantum dots;    dibutyl phthalate;   

DOI  :  10.1080/09540105.2019.1649371

来源: DOAJ

【 摘 要 】

In this study, a direct competitive molecularly imprinted biomimetic immunoassay method was developed for determination of the plasticizer dibutyl phthalate. Water-soluble carboxyl quantum dots were used as the marker. A hydrophilic molecularly imprinted polymer synthesized directly on the surface of a 96-well plate was used as biomimetic antibody. Under the optimal conditions, the limit of detection and the sensitivity of the method were 0.011 and 0.136 mg/L respectively. The cross-reaction values of two structural analogs were 4.75% and 6.89%, this indicaties that the method has the good specificity. Edible oil samples spiked with dibutyl phthalate were assayed, and good recoveries ranging from 80.0% to 111.5% were obtained. The method was used to detect dibutyl phthalate residues in liquors, and there were no significant differences obtained using the developed method and those obtained using gas chromatography.

【 授权许可】

Unknown