In vitro and in vivo evaluation of thermosensitive chitosan hydrogel for sustained release of insulin

Drug Delivery

Farzaneh Ghasemi Tahrir¹  Fariba Ganji¹  Ali Reza Mani²  Elham Khodaverdi³ 

[1] Biomedical Engineering Group, Department of Chemical Engineering;Department of Physiology, Tarbiat Modares University, Tehran, Iran, an;Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Ira;

关键词: β-glycerolphopshate;    diabetic mice;    in situ;    injectable;    subcutaneous injection;   

DOI  :  10.3109/10717544.2014.932861

来源: publisher

【 摘 要 】

Injectable In situ gel-forming chitosan/β-glycerol phosphate (CS/β-Gp) solution can be introduced into the body in a minimally invasive manner prior to solidifying within the target tissue. This hydrogel is a good candidate for achieving a prolonged drug delivery system for insulin considering its high molecular weight. In addition to the physicochemical characterization of this hydrogel, in vitro and in vivo applications were studied as a sustained insulin delivery system. In the in vitro release studies, 19–63% of total insulin was released from the CS/β-Gp hydrogel within 150 h at different β-Gp and insulin concentrations. The best formulation was selected for in vivo experimentation to control the plasma glucose of diabetic mice models. The hypoglycemic effect of this formulation following subcutaneous injection in diabetic mice lasted 5 d, significantly longer than that of free insulin solution which lasted several hours.

【 授权许可】

Unknown