Increase in the Deposition of Aggregated Protein in the Glomeruli of Spontaneously Diabetic Mice

Journal of Pharmacological Sciences

Kohei Kyuki²  Tohru Toyoshi²  Shoko Ohtsu²  Yasushi Hirasawa¹  Kazusuke Yamane²  Yoshio Suzuki¹  Yukari Matsui²  Tadashi Nagamatsu¹ 

[1] Department of Pharmacology, Faculty of Pharmacy, Meijo University;Nihon Bioresearch Inc.

关键词: diabetic mice;    diabetic nephropathy;    glomeruli;    aggregated protein;    hyperglycemia;   

DOI  :  10.1254/jphs.FPJ04052X

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(32)Cited-By(5)The aim of this study was to investigate the disposal of aggregated protein in the glomeruli of spontaneously diabetic mice. Diabetic mice, KK-Ay and db/db, and age-matched ICR mice were injected intravenously with aggregated bovine serum albumin (a-BSA) at 0.6 mg/g, and the glomeruli and the blood were obtained. Diabetic mice had larger amounts of a-BSA in their glomeruli than the ICR mice, threefold in KK-Ay and twofold in db/db, at 3 h after the a-BSA injection. Additionally, the disappearance of a-BSA was retarded in the diabetic glomeruli. KK-Ay displayed a-BSA in the glomeruli 24 h after the a-BSA injection and db/db did after 12 h, while the ICR did by 8 h. In spite of increases of insulin to similar degrees in both strains of diabetic mice after the a-BSA injection, blood glucose levels markedly decreased in KK-Ay compared with db/db. There were no histopathological alterations in the glomeruli of the diabetic mice. Depositions of a-BSA were confirmed to be higher in the diabetic glomeruli by the immunofluoresnce technique, and KK-Ay displayed higher depositions of a-BSA than did db/db. The present study suggests that hyperglycemia is involved in the increased deposition of aggregated protein in the glomeruli and that the degradation of aggregated protein is retarded in diabetic glomeruli.

【 授权许可】

Unknown