Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China

BMC Infectious Diseases

Gang Xu¹  Wen-Ping Guo¹  En-Min Zhou¹  Yi-Han Wang¹  Bing Zhang²  Xuebing Ni³  Xiaoquan Wang⁴ 

[1] 0000 0004 1760 4150, grid.144022.1, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Xinong Road 22, 712100, Yangling, Shaanxi, China;0000 0004 0369 6250, grid.418524.e, Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture, Yangling, Shaanxi, China;0000 0004 1799 3993, grid.13394.3c, Department of Human parasitology, Preclinical Medicine College, Xinjiang Medical University, Urumqi, Xinjiang, China;0000000121742757, grid.194645.b, State Key laboratory of Emerging infectious disease, School of Public Health, The University of Hong Kong, Hong Kong, China;Xuwang Town Comprehensive Agricultural Service Station, Hanzhong, Shaanxi, China;

关键词: Anaplasma capra;    Anaplasma platys;    China;    Rhipicephalus microplus;    Rrs;    gltA;    groEL;   

DOI  :  10.1186/s12879-019-4075-3

来源: publisher

【 摘 要 】

BackgroundFour species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China.MethodsHard ticks were collected and identified using morphological and molecular methods. Human-pathogenic Anaplasma species were tested using nested polymerase chain reaction. The nearly complete rrs, gltA, and groEL genes sequences from revealed Anaplasma species were amplified and sequenced to determine their molecular characteristics and their phylogeny.ResultsAll ticks collected in Ankang belonged to the Rhipicephalus microplus. Novel unclassified Anaplasma strains genetically related to A. platys and A. capra were detected in these ticks. Co-infection of these two organisms was also found. The novel unclassified Anaplasma strains identified in this study formed a distinct phylogenetic lineage based on the groEL gene and two lineages based on the gltA gene within A. platys and related strains group. The revealed A. capra strains identified in this study were most closely related to those detected in humans and other vertebrate animals.ConclusionWe revealed the presence of A. capra, a novel human pathogens in R. microplus ticks in previously unrecognized endemic regions. We also detected a novel unclassified Anaplasma species genetically related to A. platys. The epidemiology of anaplasmosis caused by these two Anaplasma species in humans should be assessed in future studies.

【 授权许可】

CC BY