Nasal delivery of H5N1 avian influenza vaccine formulated with GenJet™ or in vivo-jetPEI® induces enhanced serological, cellular and protective immune responses

Drug Delivery

Suresh K. Mittal¹  Wadzanai P. Mboko¹  James McCoy²  Weiping Cao³  Suryaprakash Sambhara³  Shivaprakash Gangappa³  Samuel Amoah⁴  Margarita Mishina⁴  Caitlin Bohannon⁵ 

[1] Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA;Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, US;Immunology and Pathogenesis Branch, National Center for Immunization and Respiratory Diseases, Atlanta, GA, USA;Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, USA;Immunology and Pathogenesis Branch, National Center for Immunization and Respiratory Diseases, Atlanta, GA, USA;Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, USA;Battelle Memorial Institute, Atlanta, GA, USA;Immunology and Pathogenesis Branch, National Center for Immunization and Respiratory Diseases, Atlanta, GA, USA;Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA, USA;Oak Ridge Institute for Science and Education (ORISE), CDC Fellowship Program, Oak Ridge, TN, USA;

关键词: Cationic polymers;    H5N1 vaccine;    adaptive immunity;    protective immunity;    mouse model;    influenza;   

DOI  :  10.1080/10717544.2018.1450909

来源: publisher

【 摘 要 】

Avian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delivery strategies are in urgent need to reduce the high risk of avian influenza pandemics. Cationic polymers have been widely used as vectors for gene delivery in vitro and in vivo. In this study, we formulated H5N1 influenza vaccines with GenJet™ or in vivo-jetPEI®, and showed that these formulations significantly enhanced the immunogenicity of H5N1 vaccines and conferred protective immunity in a mouse model. Detailed analyses of adaptive immune responses revealed that both formulations induced mixed TH1/TH2 antigen-specific CD4 T-cell responses, antigen-specific cytotoxic CD8 T-cell and memory B-cell responses. Our findings suggest that cationic polymers merit future development as potential adjuvants for mucosal delivery of poorly immunogenic vaccines.

【 授权许可】

CC BY