Replacing water and nutrients for ethanol production by ARTP derived biogas slurry tolerant Zymomonas mobilis strain

Biotechnology for Biofuels

Bo Wu¹  Han Qin¹  Guowei Duan²  Qiong Tan²  Yonghua Dai²  Furong Tan²  Weiting Wang²  Mingxiong He²  Guoquan Hu²  Yao Qin³ 

[1] Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs) Biogas Institute of Ministry of Agriculture and Rural Affairs, Section 4-13, Renmin Rd. South, 610041, Chengdu, People’s Republic of China;Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs) Biogas Institute of Ministry of Agriculture and Rural Affairs, Section 4-13, Renmin Rd. South, 610041, Chengdu, People’s Republic of China;0000 0001 0526 1937, grid.410727.7, Graduate School of Chinese Academy of Agricultural Science, 100081, Beijing, People’s Republic of China;Biomass Energy Technology Research Centre, Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs) Biogas Institute of Ministry of Agriculture and Rural Affairs, Section 4-13, Renmin Rd. South, 610041, Chengdu, People’s Republic of China;0000 0004 1798 8975, grid.411292.d, College of Pharmacy and Biological Engineering, Chengdu University, 610041, Chengdu, People’s Republic of China;

关键词: Ethanol production;    Biogas slurry;    Zymomonas mobilis;    ARTP mutagenesis;    Adaptive laboratory evolution;   

DOI  :  10.1186/s13068-019-1463-2

来源: publisher

【 摘 要 】

BackgroundReducing fresh water consumption and nutrient addition will be an effective way to reduce the whole cost of bioethanol production. On the other hand, treatment of biogas slurry derived from anaerobic digestion (AD), in which a great amount of nutrients is still left, costs too much to remove these pollutants. It would be beneficial for both digestate valorization and ethanol production if biogas slurry is used for producing bioethanol. However, both hyperosmosis and potential biotoxic components of the biogas slurry can severely inhibit fermentation.ResultsIn this study, two rounds of atmospheric and room temperature plasma (ARTP) mutagenesis combined with adaptive laboratory evolution (ALE) were applied to improve the adaptability and genetic stability of Zymomonas mobilis in biogas slurry. Mutants D95 and S912 were identified. Growth of the mutants was remarkably improved in biogas slurry. The highest ethanol productivity reached 0.63 g/L/h which was 61.7% higher than ZM4 (0.39 g/L/h). Genomic re-sequencing results also revealed that single nucleic variations (SNVs) and Indels occurred in the mutants, which are likely related to inhibitor in biogas slurry and low pH tolerance.ConclusionsOur study demonstrated that these mutant strains have great potential to produce ethanol using biogas slurry to replace fresh water and nutrients.

【 授权许可】

CC BY