Therapeutic effects of stem cells from human exfoliated deciduous teeth on diabetic peripheral neuropathy

Diabetology & Metabolic Syndrome

Jingzhi Li¹  Yuming Zhao¹  Yue Zhai¹  Lihong Ge¹  Yuanyuan Wang¹  Nanquan Rao¹  Jing Xie² 

[1] 0000 0001 2256 9319, grid.11135.37, Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, #22 Zhongguancun Nandajie, Haidian District, 100081, Beijing, China;0000 0001 2256 9319, grid.11135.37, Department of Pediatric Dentistry, School and Hospital of Stomatology, Peking University, #22 Zhongguancun Nandajie, Haidian District, 100081, Beijing, China;0000 0004 1806 5224, grid.452787.b, Department of Stomatology, Shenzhen Children’s Hospital, #7019, Yitian Road, 518026, Shenzhen, China;

关键词: Stem cells from human exfoliated deciduous teeth;    Diabetic peripheral neuropathy;    Goto-Kakizaki rats;    Stem cell therapy;   

DOI  :  10.1186/s13098-019-0433-y

来源: publisher

【 摘 要 】

ObjectiveTo evaluate the therapeutic potential of stem cells from human exfoliated deciduous teeth (SHED) for diabetic peripheral neuropathy.MethodsThe biological characteristics of SHED were identified by flow cytometric study and evaluation of differentiation potential. Using high-fat feeding, diabetes was induced in GK rats, and SHED were transplanted into the caudal veins of these rats. Immunohistochemical analysis was used to compare the capillary to muscle fiber ratio and intra-epidermal nerve fiber density between SHED- and saline-treated diabetic rats. Further, the expressions of angiogenesis-related and neurotrophic factors were quantified by real-time PCR and western blot.ResultsSHED had a capacity of multiple differentiation and shared typical characteristics of mesenchymal stem cells. SHED transplantation relieved diabetic neuropathic pain, enabled functional recovery of the peripheral nerves, and increased the capillary to muscle fiber ratio and intra-epidermal nerve fiber density compared to the saline group and normal controls. Real-time PCR results showed that the expressions of CD31, vWF, bFGF, NGF, and NT-3 in the skeletal muscles were higher in the SHED group than in the saline groups. Western blot results indicated that the levels of the CD31 and NGF proteins were higher in the SHED transplantation group than the saline group.ConclusionSHED transplantation ameliorated diabetic peripheral neuropathy in diabetic GK rats. Thus, systemic application of SHED could be a novel strategy for the treatment of diabetic peripheral neuropathy.

【 授权许可】

CC BY