The effects of vitamin-rich carbohydrate pretreatment on the surgical stress response and S-100β after splenectomy in elderly rats

BMC Anesthesiology

Lei Zhao¹  Qiuyan Yang²  Tiande Yang³  Youbo Zuo⁴  Xueli Chen⁵  Mei Zeng⁶ 

[1] 0000 0004 1758 177X, grid.413387.a, Department of Anesthesiology, Affiliated Hospital of North Sichuan Medical College, 637000, Nanchong, China;0000 0004 1758 177X, grid.413387.a, Department of Emergency, Affiliated Hospital of North Sichuan Medical College, 637000, Nanchong, China;0000 0004 1762 4928, grid.417298.1, Department of Anesthesiology, Xinqiao Hospital, Army Medical University (Third Military Medical University), 400037, Chongqing, China;0000 0004 1762 4928, grid.417298.1, Department of Anesthesiology, Xinqiao Hospital, Army Medical University (Third Military Medical University), 400037, Chongqing, China;0000 0004 1758 177X, grid.413387.a, Department of Anesthesiology, Affiliated Hospital of North Sichuan Medical College, 637000, Nanchong, China;0000 0004 1798 4472, grid.449525.b, Department of Anesthesiology, North Sichuan Medical College, 637007, Nanchong, China;0000 0004 1798 4472, grid.449525.b, Department of Genetics, School of Basic Medical Science, North Sichuan Medical College, 637007, Nanchong, China;

关键词: Carbohydrate;    Insulin resistance;    Surgical stress response;    Inflammatory mediators;    S-100β;   

DOI  :  10.1186/s12871-019-0748-0

来源: publisher

【 摘 要 】

BackgroundPreoperative oral carbohydrates has been suggested to attenuate insulin resistance and decrease postoperative complications. In this study, a vitamin-rich carbohydrate beverage was administered before surgery in an animal model to investigate its effects on the surgical stress response and S-100β levels.MethodsThirty aged male Sprague-Dawley rats were randomly assigned to three groups: control group (n = 6), fasting group (n = 12), and carbohydrate-treated group (CHO group, n = 12). Rats in the control group were not given any treatment. Rats in the fasting group received splenectomy after 12 h of fasting. In the CHO group, rats were given 5 ml of vitamin-rich carbohydrate by gavage 2 h before surgery. Fasting plasma glucose, insulin, insulin resistance (HOMA-IR index, IRI), the S-100β protein level, and the inflammatory mediators IL-1β, IL-6 and TNF-α were assessed after surgery (postoperative day (POD) 1 and 3).ResultsPostoperative insulin resistance was significantly greater in the fasting group than in the control and CHO group. The median plasma S-100β level was significantly higher in the fasting group than in the control and CHO groups on POD 1. The median plasma IL-1β level was significantly lower in the CHO group than in the fasting group on POD 1; however, no other differences in the concentrations of immunological biomarkers of stress were found between the fasting group and the CHO group.ConclusionsVitamin-rich carbohydrate pretreatment attenuated the metabolic aspect of the surgical stress response and decreased the level of plasma S-100β, which may decrease the risk of postoperative complications in elderly rats.

【 授权许可】

CC BY