Identification of key genes and molecular mechanisms associated with low egg production of broiler breeder hens in ad libitum

BMC Genomics

Zehui Wei¹  Purevsuren Lkhagvagarav¹  Cunling Jia¹  Mengqi Zhu¹  Sijia Huang¹  Pengcheng Li¹  Chuanyu Liang¹ 

[1] 0000 0004 1760 4150, grid.144022.1, College of Animal Science and Technology, Northwest A&F University, 22# Xinong Road, Yangling, Shaanxi, China;

关键词: Transcriptome;    Gallus gallus;    Ad libitum;    Restricted feeding;    Liver;    Adipose;    Ovary;   

DOI  :  10.1186/s12864-019-5801-3

来源: publisher

【 摘 要 】

BackgroundOverfeeding reduces laying performance in broiler breeder hens, which is associated with obesity, hepatic steatosis and systemic inflammation. To unravel the underlying mechanisms governing the effect of feeding regimes on energy metabolism and egg production, a transcriptomics approach was carried out for screening differentially expressed genes (DEGs) in ovary, liver and adipose tissues of broiler chickens under ad libitum and restricted feeding.ResultsIt showed that 289, 388 and 204 DEGs were identified in the adipose, liver and ovary, respectively. These DEGs were significantly enriched in phagosome pathway, lipid transport, activity and nutrient reservoir activity in ovary; steroid hormone biosynthesis and metabolism of xenobiotics by cytochrome P450 pathways in adipose tissue; and the metabolic pathways, peroxisome proliferator-activated receptor (PPAR) and Jak-STAT signaling pathway in liver. Estrogen receptor 1, identified as one of important hubs by constructing PPI network, was up-regulated in ad libitum group, which would make more apolipoproteins be transferred to ovary.ConclusionsHigh expression of VTGs, APOB, CYBB and CTSS in ovary would induce excess lipid deposit, oxidative stress and potential damage to ovulation. Our results contribute to understanding effects of feeding regimes on metabolic regulation during egg production of broiler breeder hens and also provide new evidence of metabolic regulation from integrated multi-tissue processes.

【 授权许可】

CC BY