Poly(3-hydroxybutyrate-CO-3-hydroxyvalerate) PHBHV biocompatible nanocarriers for 5-FU delivery targeting colorectal cancer

Drug Delivery

Ionut Cristian Radu¹  Catalin Zaharia¹  Horia Iovu¹  Marieta Costache²  Ariana Hudita²  Bianca Galateanu²  Kelly Velonia³  Mikhail Shtilman⁴  Octav Ginghina⁵  Aristidis Tsatsakis⁶  Carolina Negrei⁷  Sabina Georgiana Nitu⁸  Eugenia (Vasile) Tanasa⁹ 

[1] Advanced Polymer Materials Group, University Politehnica of Bucharest, Bucharest, Romania;Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania;Department of Materials Science and Technology, University of Crete, Heraklion, Greece;Department of Polymers, D.I. Mendeleyev University of Chemical Technology, Moscow, Russi;Department of Surgery, Sf. Ioan Emergency Clinical Hospital, Bucharest, Romania;Department II, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy Bucharest, Bucharest, Romania;Department of Toxicology and Forensic Sciences, Faculty of Medicine, University of Crete, Heraklion, Greece;Department of Toxicology, Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Bucharest;National Research and Development Institute for Chemistry and Petrochemistry – ICECHIM, Bucharest, Romania;University Politehnica of Bucharest, Bucharest, Romania;

关键词: Nanocarrier;    5-FU;    polyhydroxyalkanoate;    HT-29;    colorectal cancer;    anticancer efficacy;   

DOI  :  10.1080/10717544.2019.1582729

来源: publisher

【 摘 要 】

Aiming to address the issue of poor bioavailability of most anti-tumor medicines against colorectal cancer, we developed a targeted anticancer nanocarrier using biocarriers able to both bind and easily release their load in a controlled manner. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) carriers were obtained via the emulsification-diffusion method, loaded with 5-fluorouracil and then characterized in terms of particle morphology and size (SEM, DLS), drug uptake and release. The cytotoxic potential of the 5-fluorouracil-loaded polymer nanocarriers on human adenocarcinoma cells (HT-29 cell line) was investigated. The in vitro studies clearly demonstrated that while the nanocarriers themselves slightly alter HT-29 cell viability, when loaded with 5-fluorouracil they significantly decrease cell viability, suggesting that the polymer itself exhibits low cytotoxicity and the drug-loaded carrier acts in an efficient manner to kill HT-29 human adenocarcinoma cells.

【 授权许可】

CC BY