The greater effect of high-intensity interval training versus moderate-intensity continuous training on cardioprotection against ischemia-reperfusion injury through Klotho levels and attenuate of myocardial TRPC6 expression

BMC Cardiovascular Disorders

Maral Ramez¹  Hamid Rajabi¹  Fatemeh Ramezani²  Farinaz Nasirinezhad²  Amir Darbandi-Azar³  Nasim Naderi³ 

[1] 0000 0004 0406 5813, grid.412265.6, Department of Exercise physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran;0000 0004 4911 7066, grid.411746.1, Physiology Research Center and Physiology Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran;0000 0004 4911 7066, grid.411746.1, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran;

关键词: Ischemia-reperfusion injury;    Cardioprotection;    Exercise training;    Klotho;    TRPC6;   

DOI  :  10.1186/s12872-019-1090-7

来源: publisher

【 摘 要 】

BackgroundMyocardial ischemia-reperfusion (IR) injury is a leading cause of death all over the world, so developing practical approaches to promote cardioprotection against IR injury is essential. Exercise training is an effective strategy to improve cardioprotection. Hence, the purpose of this study was to investigate the effect of short-term preconditioning with two types of high-intensity interval training (HIIT) and moderate intensity continuous training (MICT) on klotho and TRPC6 mechanisms in cardioprotection.MethodsEighty Male Wistar rats (250–300 g) were randomly divided into 7 groups, including Control, HIIT, MICT, Sham, IR, HIIT+IR, and MICT+IR. Training was performed in 5 consecutive days. HIIT protocol consisted of running on the treadmill at intervals 85–90% vo2max that separated by slow intensity periods at 50–60% vo2max. MICT program was performed at 70% VO2max at the same running distance with HIIT groups. The cardiac IR injury was induced by LAD occlusion followed by reperfusion. ELISA kit was used in order to measure the plasma levels of klotho, LDH and CK-MB, and TRPC6 expression was determined using the western blot technique. Data were analyzed using one way ANOVA and Tukey’s post hoc tests.ResultsThe results of this study showed that both types of exercise training programs significantly increase plasma levels of klotho and reduce the infarct size and heart injury. In addition, the exercise training decreased the amount of TRPC6 channels expression during IR. However, the effect of HIIT on increasing the klotho and cardioprotection was greater compared to MICT.ConclusionsBased on the results, even a short-term of aerobic exercise training, especially HIIT, promotes cardioprotection against IR injury and decreases infarct size via an increase in klotho and attenuate of protein expression of myocardial TRPC6 during IR.

【 授权许可】

CC BY