Comparative transcriptome analysis of trout skin pigment cells

BMC Genomics

Simona Sušnik Bajec¹  Ida Djurdjevič¹  Seita Miyazawa²  Tomasz Furmanek³ 

[1] 0000 0001 0721 6013, grid.8954.0, Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, SI-1230, Domžale, Slovenia;0000 0004 0373 3971, grid.136593.b, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan;0000 0004 0427 3161, grid.10917.3e, Institute of Marine Research, Bergen, Norway;

关键词: Salmo marmoratus;    Salmo trutta;    Pigment cell;    DEG;    RNA-seq;    qPCR;   

DOI  :  10.1186/s12864-019-5714-1

来源: publisher

【 摘 要 】

BackgroundEnormous variability in skin colour and patterning is a characteristic of teleost fish, including Salmonidae fishes, which present themselves as a suitable model for studying mechanisms of pigment patterning. In order to screen for candidate genes potentially involved in the specific skin pigment pattern in marble trout (labyrinthine skin pattern) and brown trout (spotted skin pattern), we conducted comparative transcriptome analysis between differently pigmented dermis sections of the adult skin of the two species.ResultsDifferentially expressed genes (DEGs) possibly associated with skin pigment pattern were identified. The expression profile of 27 DEGs was further tested with quantitative real-time PCR on a larger number of samples. Expression of a subset of ten of these genes was analysed in hybrid (marble x brown) trout individuals and compared with the complexity of their skin pigment pattern. A correlation between the phenotype and the expression profile assessed for hybrid individuals was detected for four (gja5, clcn2, cdkn1a and tjp1) of the ten candidate genes tested. The potential role of these genes in skin pigment pattern maintenance is discussed.ConclusionsOur results indicate that the maintenance of different pigment patterns in trout is dependent upon specific communication—involving gap junctions, tight junctions and ion channels—between chromatophores present in differentially pigmented skin regions.

【 授权许可】

CC BY