期刊论文详细信息
BMC Veterinary Research
Determination of the sensitivity and specificity of bovine tuberculosis screening tests in dairy herds in Thailand using a Bayesian approach
Sukolrat Boonyayatra1  Tawatchai Singhla1  Srinand Sreevatsan2  Julio Alvarez2  Scott J. Wells3  Songkhla Chulakasian4  Mintra Lukkana4 
[1] 0000 0000 9039 7662, grid.7132.7, Department of Food Animal Clinic and Excellent Center of Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand;0000000419368657, grid.17635.36, Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA;0000000419368657, grid.17635.36, Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA;0000000419368657, grid.17635.36, Center for Animal Health and Food Safety, University of Minnesota, St. Paul, MN, USA;National Bureau of Agricultural Commodity and Food Standards, Bangkok, Thailand;
关键词: Mycobacterium bovis;    Test performance;    Single intradermal tuberculin test;    Interferon gamma assay;    ELISA test;   
DOI  :  10.1186/s12917-019-1905-x
来源: publisher
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【 摘 要 】

BackgroundThe objective of this study was to determine the sensitivity (Se) and specificity (Sp) of bovine tuberculosis (bTB) screening tests including a single intradermal tuberculin (SIT) test, interferon gamma (IFN-γ) assay, and a commercial ELISA test (M. bovis Ab) in dairy cattle, under field conditions, using a Bayesian approach.ResultsThe study population consisted of 128 dairy cows from 25 bTB-infected herds in Chiang Mai and Chiang Rai provinces, Thailand. A single-population Bayesian model was implemented assuming conditional dependence between the SIT test and IFN-γ assays. The 95% posterior probability interval (PPI) of the SIT test (severe interpretation) Se ranged from 75.3 to 95.2% (median = 87.6%), while the Sp was slightly lower (median = 83.6%, PPI = 74.2–92.8%). The IFN-γ assay Se was moderate and the 95% PPI ranged from 38.6 to 74.4% (median = 55.7%) with higher Sp (median = 93.5.4%, PPI = 87.0–98.1%). The M. bovis Ab ELISA Se was low, with 95% PPI ranging between 30.0 and 71.2% (median = 47.4%); however, the Sp was high (median = 90.9%, PPI = 84.5–95.5%).ConclusionThe SIT test sensitivity was similar to that demonstrated in other regions and can, therefore, be used effectively as part of control programs in this area. The IFN-γ and M. bovis Ab ELISA assays can be applied as supplementary techniques. The test performance of these tests when used as single tests without confirmation, however, are expected to continue to challenge disease eradication efforts.

【 授权许可】

CC BY   

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