Development and validation of SIRT3-related nomogram predictive of overall survival in patients with serous ovarian cancer

Journal of Ovarian Research

Jun Li¹  Xin Lu¹  Huiran Yue¹  Xiaohong Xue¹  Hailin Yu¹ 

[1] 0000 0001 0125 2443, grid.8547.e, Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, No.419, Fangxie Road, 200011, Shanghai, China;Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 200011, Shanghai, China;

关键词: Ovarian cancer;    Sirtuin;    SIRT3;    Nomogram;    Prognosis;   

DOI  :  10.1186/s13048-019-0524-2

来源: publisher

【 摘 要 】

ObjectiveOur aim is to analyzed the expression pattern of sirtuin(SIRT) superfamily and evaluated their prognostic values in serous ovarian cancer patients.MethodsWe first analyzed the differential expression of SIRT members among fallopian tube epithelium (FTE), primary serous ovarian cancers/tubal cancers (PSOCs/PSTCs), and omental metastases using GSE10971 and GSE30587 datasets. The prognostic values of SIRT members were evaluated using TCGA and GSE9891 dataset.ResultsSIRT3 and SIRT5 expression were significantly decreased and increased in PSOCs/PSTCs compared with that in normal counterparts, respectively. SIRT6 and SIRT7 were overexpressed in ometal metastases compared with corresponding primary counterparts. With respect to recurrence free survival, however, SIRT7 overexpression was correlated with better prognosis. A similar trend was observed by multivariable analysis. Regarding overall survival (OS), increased expression of SIRT3, SIRT5, and SIRT7 were associated with better survival by univariable analysis. Subsequent multivariable analysis showed that SIRT3 remained an independent favorable prognostic factor for OS. The SIRT3-related nomogram illustrated age at initial diagnosis as sharing the largest contribution to OS, followed by SIRT3 expression and FIGO stage. The C-index for OS prediction was 0.65 (95%CI, 0.61–0.69) in training cohort (TCGA dataset) and 0.65 (95%CI, 0.59–0.71) in validation cohort (GSE9891 dataset), respectively. The calibration plots showed optimal agreement between the prediction by SIRT3-related nomogram and actual observation for 1-, 3-, and 5-year OS probability.ConclusionIn conclusion, SIRT3 was an independent favorable prognostic factor for OS in serous ovarian cancer, and added prognostic value to the traditional clinicopathological factors used to evaluate patients’ prognosis.

【 授权许可】

CC BY