Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes

Parasites & Vectors

Keshava Mysore¹  Longhua Sun¹  Limb K. Hapairai¹  Jacob S. Realey¹  Ping Li¹  Molly Duman-Scheel²  David W. Severson³  Nicholas D. Scheel⁴  Chien-Wei Wang⁵  Na Wei⁵ 

[1] 0000 0000 8679 3494, grid.257425.3, Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN, USA;0000 0001 2168 0066, grid.131063.6, Eck Institute for Global Health, The University of Notre Dame, Notre Dame, IN, USA;0000 0000 8679 3494, grid.257425.3, Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN, USA;0000 0001 2168 0066, grid.131063.6, Eck Institute for Global Health, The University of Notre Dame, Notre Dame, IN, USA;0000 0001 2168 0066, grid.131063.6, Department of Biological Sciences, The University of Notre Dame, Notre Dame, IN, USA;0000 0000 8679 3494, grid.257425.3, Department of Medical and Molecular Genetics, Indiana University School of Medicine, South Bend, IN, USA;0000 0001 2168 0066, grid.131063.6, Eck Institute for Global Health, The University of Notre Dame, Notre Dame, IN, USA;0000 0001 2168 0066, grid.131063.6, Department of Biological Sciences, The University of Notre Dame, Notre Dame, IN, USA;grid.430529.9, Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad, Trinidad and Tobago;0000 0001 2168 0066, grid.131063.6, Eck Institute for Global Health, The University of Notre Dame, Notre Dame, IN, USA;0000 0001 2168 0066, grid.131063.6, Department of Biological Sciences, The University of Notre Dame, Notre Dame, IN, USA;0000 0001 2168 0066, grid.131063.6, Eck Institute for Global Health, The University of Notre Dame, Notre Dame, IN, USA;0000 0001 2168 0066, grid.131063.6, Department of Civil and Environmental Engineering and Earth Sciences, The University of Notre Dame, Notre Dame, IN, USA;

关键词: Aedes aegypti;    Anopheles gambiae;    Culex quinquefasciatus;    Saccharomyces cerevisiae;    Arbovirus;    Dengue;    Malaria;    Insecticide;    RNA interference;    Nervous system;   

DOI  :  10.1186/s13071-019-3504-x

来源: publisher

【 摘 要 】

BackgroundRNA interference (RNAi), which has facilitated functional characterization of mosquito neural development genes such as the axon guidance regulator semaphorin-1a (sema1a), could one day be applied as a new means of vector control. Saccharomyces cerevisiae (baker’s yeast) may represent an effective interfering RNA expression system that could be used directly for delivery of RNA pesticides to mosquito larvae. Here we describe characterization of a yeast larvicide developed through bioengineering of S. cerevisiae to express a short hairpin RNA (shRNA) targeting a conserved site in mosquito sema1a genes.ResultsExperiments conducted on Aedes aegypti larvae demonstrated that the yeast larvicide effectively silences sema1a expression, generates severe neural defects, and induces high levels of larval mortality in laboratory, simulated-field, and semi-field experiments. The larvicide was also found to induce high levels of Aedes albopictus, Anopheles gambiae and Culex quinquefasciatus mortality.ConclusionsThe results of these studies indicate that use of yeast interfering RNA larvicides targeting mosquito sema1a genes may represent a new biorational tool for mosquito control.

【 授权许可】

CC BY