Evaluation of the anticancer potential of a sulphonamide carbonic anhydrase IX inhibitor on cervical cancer cells

Journal of Enzyme Inhibition and Medicinal Chemistry

Ismail Koyuncu¹  Mustafa Durgun²  Hewa Slahaddin Qadir³  Yasin Tülüce³  Claudiu T. Supuran⁴ 

[1] Department of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey;Department of Chemistry, Faculty of Arts and Sciences, Harran University, Sanliurfa, Turkey;Department of Medical Biology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey;Neurofarba Department, Section of Pharmaceutical and Nutriceutical Sciences, Università degli Studi di Firenze, Sesto Fiorentino (Florence), Ital;

关键词: Apoptosis;    cytotoxicity;    cervical cancer;    carbonic anhydrase-IX inhibitor;    oxidative stress;   

DOI  :  10.1080/14756366.2019.1579805

来源: publisher

【 摘 要 】

Cervical cancer is a common type of cancer. Carbonic anhydrase IX (CA IX) is an attractive target for tumour therapy, being overexpressed in many cancers. We investigated the anticancer properties of the aromatic sulphonamide S-1 as a CA IX inhibitor on cervical cancer cells (HeLa) positive for CA IX expression and normal prostate epithelial cell line (PNT1-A) negative for CA IX. We examined the cytotoxic, apoptosis, genotoxic, and oxidative stress activity of S-1 on HeLa and PNT1-A cell lines. S-1 induced significant reduction of cell viability, caused apoptosis, and up-regulated ROS production. This decrease in cell survival rate can be attributed to the high level of ROS and apoptosis, which has also been shown to arrest the cell cycle. Our findings indicated that S-1 is more effective on HeLa than PNT1-A. S-1 was able to induce apoptosis of cervical cancer cells and is a possible candidate for future anticancer studies.

【 授权许可】

CC BY