Human embryonic stem cell-derived exosomes promote pressure ulcer healing in aged mice by rejuvenating senescent endothelial cells

Stem Cell Research & Therapy

Zhifeng Deng¹  Qingwei Zhu¹  Yongjin Sun²  Bi Chen²  Qing Li³  Yunlong Yang³  Yang Wang³  Xin Niu³  Juntao Zhang³ 

[1] 0000 0004 1798 5117, grid.412528.8, Department of Neurosurgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233, Shanghai, China;0000 0004 1798 5117, grid.412528.8, Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233, Shanghai, China;0000 0004 1798 5117, grid.412528.8, Institute of Microsurgery on Extremities, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 200233, Shanghai, China;

关键词: Senescence;    Angiogenesis;    Exosomes;    Embryonic stem cells;    Nrf2;   

DOI  :  10.1186/s13287-019-1253-6

来源: publisher

【 摘 要 】

BackgroundAngiogenesis, as an endogenous repair mechanism, plays crucial roles in wound healing and tissue regeneration. However, this process is impaired in the elderly due to aging-related vascular endothelial dysfunction. This study was aimed to explore the pro-angiogenic effects of exosomes from human embryonic stem cells (ESC-Exos) in aged mice of pressure-induced ulcer model and the underlying mechanism.MethodsPressure ulcer wounds were created on the back of d-galactose-induced aging mice. ESC-Exos were locally applied onto the wound beds, with PBS as control. The effects of ESC-Exos on wound healing were analyzed by measuring wound closure rates, histological and immunofluorescence analyses. Then, the anti-aging effect of ESC-Exos on vascular endothelial cells was tested in an in vitro d-galactose-induced HUVEC senescence model.ResultsESC-Exos could accelerate wound closure and enhance angiogenesis, and the senescence of vascular endothelial cells was significantly ameliorated after ESC-Exos treatment. In vitro, ESC-Exos could rejuvenate the senescence of endothelial cells and recover compromised proliferation, migratory capacity, and tube formation. This recovery was Nrf2-activation-dependent, since cotreatment with Nrf2 inhibitor Brusatol could abolish the rejuvenative effects of ESC-Exos. Further study revealed that miR-200a was highly enriched in ESC-Exos and played a crucial role in ESC-Exos-mediated rejuvenation through downregulating Keap1, which negatively regulates Nrf2 expression.ConclusionsESC-Exos ameliorate endothelial senescence by activating Nrf2 and recover aging-related angiogenic dysfunction, thereby accelerating wound healing in aged mice. ESC-Exos might be a natural nano-biomaterial for aging-related diseases therapy.

【 授权许可】

CC BY