Identification and characterization of the α-CA in the outer membrane vesicles produced by Helicobacter pylori

Journal of Enzyme Inhibition and Medicinal Chemistry

Sonia Del Prete¹  Clemente Capasso¹  Vincenzo Carginale¹  Maurizio Ronci²  Valentina Puca³  Rossella Grande⁴  Gabriella Mincione⁵  Antonio Aceto⁵  Raffaella Muraro⁵  Simone Carradori⁶  Francesca Sisto⁷  Claudiu T. Supuran⁸ 

[1] CNR, Istituto di Bioscienze e Biorisorse, Napoli, Italy;CeSI-MeT Centro Scienze dell’Invecchiamento e Medicina Traslazionale, Center of Aging Sciences and Translational Medicine (CeSi-Met), Chieti, Italy;Department of Medical, Oral, and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Chieti, Italy;CeSI-MeT Centro Scienze dell’Invecchiamento e Medicina Traslazionale, Center of Aging Sciences and Translational Medicine (CeSi-Met), Chieti, Italy;Department of Medicine and Aging Science, G. d’Annunzio of Chieti-Pescara, Chieti, Italy;CeSI-MeT Centro Scienze dell’Invecchiamento e Medicina Traslazionale, Center of Aging Sciences and Translational Medicine (CeSi-Met), Chieti, Italy;Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Chieti, Italy;Department of Medical, Oral, and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, Chieti, Italy;Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, Chieti, Italy;Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, University of Milan, Milan, Italy;NEUROFARBA Department, Sezione di Scienze Farmaceutiche e Nutraceutiche, Università degli Studi di Firenze, Sesto, Ital;

关键词: Carbonic anydrases;    Helicobacter pylori;    outer membrane vesicles (OMVs);    biofilm;    protonography;    mass spectrometry;   

DOI  :  10.1080/14756366.2018.1539716

来源: publisher

【 摘 要 】

The genome of Helicobacter pylori encodes for carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the α- and β-CA classes, which together with urease, have a pivotal role in the acid acclimation of the microorganism within the human stomach. Recently, in the exoproteome of H. pylori, a CA with no indication of the corresponding class was identified. Here, using the protonography and the mass spectrometry, a CA belonging to the α-class was detected in the outer membrane vesicles (OMVs) generated by planktonic and biofilm phenotypes of four H. pylori strains. The amount of this metalloenzyme was higher in the planktonic OMVs (pOMVs) than in the biofilm OMVs (bOMVs). Furthermore, the content of α-CA increases over time in the pOMVs. The identification of the α-CA in pOMVs and bOMVs might shed new light on the role of this enzyme in the colonization, survival, persistence, and pathogenesis of H. pylori.

【 授权许可】

CC BY