期刊论文详细信息
BMC Microbiology | |
The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B1 | |
Ningning Song1  Zhaoli Li1  Liping Chen1  He Li1  Yingying Cui1  Zhe Li1  Guanghui Dang1  Siguo Liu1  Ziyin Cui1  | |
[1]0000 0001 0526 1937, grid.410727.7, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China | |
关键词: Transcriptomics; Metabolomics; BCG; Vitamin B; Growth inhibition; | |
DOI : 10.1186/s12866-019-1492-9 | |
来源: publisher | |
【 摘 要 】
BackgroundVitamin B1 (VB1) is a crucial dietary nutrient and essential cofactor for several key enzymes in the regulation of cellular and metabolic processes, and more importantly in the activation of immune system. To date, the precise role of VB1 in Mycobacterium tuberculosis remains to be fully understood.ResultsIn this study, the transcriptional and metabolic profiles of VB1-treated Mycobacterium. bovis BCG were analyzed by RNA-sequencing and LC-MS (Liquid chromatography coupled to mass spectrometry). The selection of BCG strain was based on its common physiological features shared with M. tuberculosis. The results of cell growth assays demonstrated that VB1 inhibited the BCG growth rate in vitro. Transcriptomic analysis revealed that the expression levels of genes related to fatty acid metabolism, cholesterol metabolism, glycolipid catabolism, DNA replication, protein translation, cell division and cell wall formation were significantly downregulated in M. bovis BCG treated with VB1. In addition, the metabolomics LC-MS data indicated that most of the amino acids and adenosine diphosphate (ADP) were decreased in M. bovis BCG strain after VB1 treatment.ConclusionsThis study provides the molecular and metabolic bases to understand the impacts of VB1 on M.bovis BCG.【 授权许可】
CC BY
【 预 览 】
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