A roadmap for gene functional characterisation in crops with large genomes: Lessons from polyploid wheat

eLife

William D Bovill¹  Brett Ford¹  Ben Trevaskis¹  Luigi Cattivelli²  Curtis J Pozniak³  Marco Maccaferrri⁴  Julie King⁵  Bruno Contreras-Moreira⁶  Guy Naamati⁶  Carolina Sansaloni⁷  Alison R Bentley⁸  James Cockram⁸  Jemima Brinton⁹  Sadiye Hayta⁹  Ricardo H Ramirez-Gonzalez⁹  Clémence Marchal⁹  Wendy Harwood⁹  Nikolai M Adamski⁹  Brande BH Wulff⁹  Sophie A Harrington⁹  Cristobal Uauy⁹  Luzie U Wingen⁹  Sreya Ghosh⁹  Lee T Hickey^1,10  Keywan Hassani-Pak^1,11  Kostya Kanyuka^1,11  Philippa Borrill^1,12 

[1] Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food (CSIRO), Canberra, Australia;Council for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics, Fiorenzuola d'Arda, Italy;Crop Development Centre, University of Saskatchewan, Saskatoon, Canada;Department of Agricultural and Food Sciences (DISTAL), Alma Mater Studiorum - Università di Bologna (University of Bologna), Bologna, Italy;Division of Plant and Crop Sciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, United Kingdom;European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, United Kingdom;International Maize and Wheat Improvement Center (CIMMYT), El Batán, Mexico;John Bingham Laboratory, Cambridge, United Kingdom;John Innes Centre, Norwich Research Park, Norwich, United Kingdom;Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Australia;Rothamsted Research, Harpenden, United Kingdom;School of Biosciences, University of Birmingham, Birmingham, United Kingdom;

关键词: crop genetics;    genomics;    wheat;    polyploidy;   

DOI  :  10.7554/eLife.55646

来源: publisher

【 摘 要 】

Understanding the function of genes within staple crops will accelerate crop improvement by allowing targeted breeding approaches. Despite their importance, a lack of genomic information and resources has hindered the functional characterisation of genes in major crops. The recent release of high-quality reference sequences for these crops underpins a suite of genetic and genomic resources that support basic research and breeding. For wheat, these include gene model annotations, expression atlases and gene networks that provide information about putative function. Sequenced mutant populations, improved transformation protocols and structured natural populations provide rapid methods to study gene function directly. We highlight a case study exemplifying how to integrate these resources. This review provides a helpful guide for plant scientists, especially those expanding into crop research, to capitalise on the discoveries made in Arabidopsis and other plants. This will accelerate the improvement of crops of vital importance for food and nutrition security.

【 授权许可】

CC BY