An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

Molecules

Markus Kurz¹  Guannan Kuang¹ 

[1] Phylos, Inc., 128 Spring Street, Lexington, MA 02421, USA

关键词: Nucleic acid-encoded libraries;    mRNA-protein fusion;    PROfusionTM;    in vitro selection;    puromycin;    psoralen;   

DOI  :  10.3390/51201259

来源: mdpi

【 摘 要 】

We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.