User of Methomyl insecticide for the selection of spot blotch resistant oat genotypes

Ciência Rural

Barbieri, Rosa Lia¹  Dornelles, Ana Lúcia Cunha¹  Milach, Sandra Cristina Kothe²  Bered, Fernanda¹  Federizzi, Luiz Carlos¹  Handel, Cristine Luise³ 

[1] Universidade Federal do Rio Grande do Sul;Universidade Federal do Rio Grande do Sul, Porto Alegre;University of Minnesota, EUA

关键词: Avena sativa;    in vitro selection;    Helminthosporium sativum;    Methomyl;   

DOI  :  10.1590/S0103-84781999000200003

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

Spot Blotch is a plant disease which causes yield losses in oat and other cereals. Selection for resistant genotypes using Helminthosporium sativum toxic filtrates is an efficient technique, which reduces the pathogen variability and the influence of the environment over the genotype expression. The filtrates extraction is time consuming and a difficult process, and the possibility of using a synthetic product to simulate its action of inhibiting the cell electron transport chain would be useful. The Methomyl insecticide is an efficient product to simulate the effects of the fungus that causes spot blotch in corn. The objective of this study was to check its efficiency in oat. Oat roots and callus growth were evaluated in the insecticide presence. Growth values that were less inhibited by the insecticide were used to correlate with spot blotch resistance. The results indicate that Methomyl reduces the growth of oat root and calli and may be used to differentiate genotypes more and less resistant to Spot Blotch. Therefore, UFRGS 14, which was the most affected genotype in the presence of the fungus toxic filtrates, had also the most reduced root and calli growth in the presence of Methomyl.

【 授权许可】

CC BY-NC