The Preparation of Fluorescence-Quenched Probes for Use in the Characterization of Human Factor Xa Substrate Binding Domains

Molecules

Karen M. Bromfield¹  Julia Cianci² 

[1] Centre for Green Chemistry, Monash University, Clayton, Victoria, 3800. Australia.;School of Chemistry, Monash University, Clayton, Victoria, 3800. Australia.

关键词: Solid phase peptide synthesis;    parallel synthesis;    fluorescence-quenched substrates;    protease;    factor Xa;   

DOI  :  10.3390/90600427

来源: mdpi

【 摘 要 】

The preparation and characterization by LCMS of a library of 55 fluorescence- quenched peptides is described. The peptides bear a terminal anthranilamide fluorophore and a penultimate 2,4-dinitrophenyl-l-lysine quencher, and will be used to probe the substrate binding domain of the human blood coagulation enzyme, factor Xa.

【 授权许可】

Unknown   
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