Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection

Sensors

Dorota G. Bijanowska¹  Marek Dawgul¹ 

[1] Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Trojdena 4, 02-109 Warsaw, Poland

关键词: Urea detection;    biosensors;    EnFET;    enzyme immobilisation;   

DOI  :  10.3390/s30600160

来源: mdpi

【 摘 要 】

In this paper urea biosensors based on detection of pH and ammonium ions are presented. As transducers pH-sensitive ion-selective field effect transistors (ISFETs) and chemically modified FETs (ChemFETs) snsitive to ammonium ions were used.Results concerning urea determination by the bisensors in blood plasma and in dialysate show that the biosensors can be applied for urea monitoring in the effluent dialysate. However in the case of pNH₄ based EnFETs a special pre-treatment (dilution with tris/HCl buffer) of the samples is necessary.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.