On-line monitoring of urea using enzymatic field effect transistors

SENSORS AND ACTUATORS B-CHEMICAL

卷:160

Article

Sant, W.²  Temple-Boyer, P.^1,3  Chanie, E.²  Launay, J.^1,3  Martinez, A.^1,3 

[1] LAAS, CNRS, Toulouse, France

[2] HEMODIA SA, F-31682 Labege, France

[3] Univ Toulouse, UPS, INSA, INP,ISAE,LAAS, F-31077 Toulouse, France

关键词: ChemFET sensor;    Enzymatic reaction;    Urea detection;    Flow cell analysis;    Haemodialysis;   

DOI  :  10.1016/j.snb.2011.07.012

来源: Elsevier

【 摘 要 】

Silicon and polymer-based technologies were developed in order to reach the industrial fabrication of disposable, mass-fabricated microsensors for the urea detection in liquid phase. The detection principle is based on pH-sensitive chemical field effect transistor (pH-ChemFET) microdevices. In order to realize an urea-sensitive enzymatic field effect transistor (urea-EnFET), studies involve the integration of an urease-based enzymatic layer by ink jet printing techniques, the packaging into a specific flow cell compatible with the liquid phase measurement, and the development of urea-EnFET/pH-ChemFET differential analysis technique. Thus, the urea detection properties were studied in EDTA-based and dialysate solutions, evidencing good sensitivity in concentration ranges appropriate to human kidney failure. Finally, the whole analysis system was successfully tested for the continuous monitoring of urea in artificial dialysis conditions. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

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