Phytochelatin Modified Electrode Surface as a Sensitive Heavy-Metal Ion Biosensor

Sensors

Vojtech Adam⁴  Josef Zehnalek⁴  Jitka Petrlova⁴  David Potesil⁴  Bernd Sures³  Libuse Trnkova⁵  Frantisek Jelen¹  Jan Vitecek² 

[1]Laboratory of Biophysical Chemistry and Molecular Oncology, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, Brno 612 65, Czech Republic

[2]Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Zemedelska 1, s611 37 Brno, Czech Republic

[3]Univ. Karlsruhe, Zool. Inst. Okol Parasitol 1, Karlsruhe, D-76128 Germany

[4]Department of Chemistry and Biochemistry University of Agriculture and Forestry, Zemedelska 1, 611 37 Brno, Czech Republic

[5]Department of Theoretical and Physical Chemistry Masaryk University Faculty of Science, Kotlarska 2, 611 37 Brno

关键词: phytochelatin;    adsorptive transfer stripping;    differential pulse voltammetry;    mercury;    cadmium;    zinc;    heavy metal sensor;    human urine;    cis-platin;   

DOI  :  10.3390/s5010070

来源: mdpi

【 摘 要 】

Electrochemical biosensors have superior properties over other existing measurement systems because they can provide rapid, simple and low-cost on-field determination of many biological active species and a number of dangerous pollutants. In our work, we suggested a new heavy metal biosensor based on interaction of heavy metal ions (Cd²⁺ and Zn²⁺) with phytochelatin, which was adsorbed on the surface of the hanging mercury drop electrode, using adsorptive transfer stripping differential pulse voltammetry. In addition, we applied the suggested technique for the determination of heavy metals in a biological sample – human urine and platinum in a pharmaceutical drug. The detection limits (3 S/N) of Cd(II), Zn(II) and cis-platin were about 1.0, 13.3 and 1.9 pmole in 5 μl, respectively. On the basis of the obtained results, we propose that the suggested technique offers simple, rapid, and low-cost detection of heavy metals in environmental, biological and medical samples.

【 授权许可】

Unknown   
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