Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

International Journal of Environmental Research and Public Health

Miguel Angel Merlos Rodrigo¹  Natalia Cernei²  Marketa Kominkova³  Ondrej Zitka¹  Miroslava Beklova²  Josef Zehnalek¹  Rene Kizek¹ 

[1]Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic

[2] E-Mails:

[3]Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic

[4] E-Mails:

[5]Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic

[6] E-Mail:

关键词: ion exchange chromatography;    mass spectrometry;    MALDI-TOF;    phytochelatin;    cadmium;    intramolecular complex;   

DOI  :  10.3390/ijerph10041304

来源: mdpi

【 摘 要 】

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL⁻¹ PC2 and 100 µg·mL⁻¹ of CdCl₂. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.