Sensors | |
A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide | |
Huai-Sheng Wang1  Qian-Xiu Pan2  | |
[1] id="af1-sensors-05-00266">Department of Chemistry, Institute of Analytical Chemistry, Liaocheng University, Liaocheng, 252059 Chi | |
关键词: horseradish peroxidase; biosensor; hydrogen peroxide; chitosan; | |
DOI : 10.3390/s5040266 | |
来源: mdpi | |
【 摘 要 】
An amperometric biosensor for hydrogen peroxide (H2O2) was developed via an easy and effective enzyme immobilization method with the “sandwich” configuration: ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basic electrode. In order to prevent the loss of immobilized HRP under optimized conditions, the biosensor surface was cross-linked with glyoxal. Ferrocene was selected and immobilized on the glassy carbon electrode surface as a mediator. The fabrication procedure was systematically optimized to improve the biosensor performance. The biosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5×10-5 to 1.1×10-3 M, and a detection limit of 8.0×10-6 M based on S/N = 3.
【 授权许可】
Unknown
© 2005 by MDPI (
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190060045ZK.pdf | 165KB | download |