A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide

Sensors

Huai-Sheng Wang¹  Qian-Xiu Pan² 

[1] id="af1-sensors-05-00266">Department of Chemistry, Institute of Analytical Chemistry, Liaocheng University, Liaocheng, 252059 Chi

关键词: horseradish peroxidase;    biosensor;    hydrogen peroxide;    chitosan;   

DOI  :  10.3390/s5040266

来源: mdpi

【 摘 要 】

An amperometric biosensor for hydrogen peroxide (H₂O₂) was developed via an easy and effective enzyme immobilization method with the “sandwich” configuration: ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basic electrode. In order to prevent the loss of immobilized HRP under optimized conditions, the biosensor surface was cross-linked with glyoxal. Ferrocene was selected and immobilized on the glassy carbon electrode surface as a mediator. The fabrication procedure was systematically optimized to improve the biosensor performance. The biosensor had a fast response of less than 10 s to H₂O₂, with a linear range of 3.5×10^-5 to 1.1×10^-3 M, and a detection limit of 8.0×10^-6 M based on S/N = 3.

【 授权许可】

Unknown   
© 2005 by MDPI (http://www.mdpi.org).