Comparative Study of Protein Immobilization Properties on Calixarene Monolayers

Sensors

Hongxia Chen⁴  Minsu Lee¹  Sungwook Choi⁵  Jae-Ho Kim⁵  Heung-Jin Choi³  Sung-Hoon Kim³  Jeabeom Lee² 

[1] Seed Biochips, Chung-Buk, 363-883, Korea; E-mail:;Department of Nanomedical Engineering, Pusan National University, Pusan 609-735, Korea; E-mail:;Department of Textile System Engineering, Kyungpook National University, Taegu 702-701, Korea; E-mail:;College of Pharmacy, Pusan National University, Pusan 609-735, Korea; E-mail:;Department of Molecular Science and Technology, Ajou University, Suwon 442-749, Korea; E-mail:

关键词: Calix[4]arene;    Self-assembled monolayer;    Linker system;    anti-hIgG;    Biosensor;   

DOI  :  10.3390/s7071091

来源: mdpi

【 摘 要 】

Three calix[4]arene (Cal-4) derivatives of which contain ethylester (1), carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at the upper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Au films. After spectroscopic characterization of monolayers, the interaction between Cal-4 and surface confined bovine serum albumin (BSA) in the SAMs was analyzed by surface plasmon resonance (SPR). The estimated surface concentration of BSA on the Cal-4 SAM with crownether group was the highest among the three Cal-4 derivatives. Anti-hIgG and hIgG pair was employed for the investigation of protein-protein interaction. Molecular interaction between anti-hIgG and hIgG can be detected in a concentration range of 10 pg/mL to 200 pg/mL on the Cal-4 derivative 3 SAM modified SPR chip.

【 授权许可】

Unknown   
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