Enhancement of BSA Binding on Au Surfaces by calix[4]bisazacrown Monolayer

Sensors

Hongxia Chen²  Youn Sook Kim¹  Jaebeom Lee¹  Seok Ju Yoon³  Dong Seob Lim³  Heung-Jin Choi³ 

[1] College of Nanoscience and Nanotechnology, Pusan National University, Pusan 609-735, Korea;College of Pharmacy, Pusan National University, Pusan 609-735, Korea;College of Engineering, Kyungpook National University, Taegu 702-701, Korea

关键词: Azacrown ether;    Self-assembled monolayer (SAM);    Linker system;    Bovine serum albumin (BSA);    Biochip;   

DOI  :  10.3390/s7102263

来源: mdpi

【 摘 要 】

Effective investigation of biomolecular structure and function with chip-based modern instruments often requires reliable and steady attachment of designated biomolecules on substrate. Here, we investigated the formation of self-assembled monolayer (SAM) with a new calix[4]arene derivative containing bisazacrown ether at the lower rim (calix[4]bisazacrown) where ammonium moieties of proteins can mainly be interacted with. Immobilization process of protein using bovine serum albumin (BSA) on the Au surface modified with calix[4]bisazacrown monolyer as an artificial linker system was monitored by surface plasmon resonance (SPR) technique. The surface concentration of BSA calculated by the simulation of SPR experimental data was higher than that of a well-known similar commercial protein linker. These results can help in modeling and understanding of protein immobilization on solid surface as well as further development lab-on-a-chip (LOC) devices for biomedical diagnosis kit of certain protein related diseases as biomarkers.

【 授权许可】

Unknown   
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