Piezoelectric Sensor for Determination of Genetically Modified Soybean Roundup Ready® in Samples not Amplified by PCR

Sensors

Magdalena Stobiecka¹  Jarosᐪw M. Cieśla²  Beata Janowska²  Barbara Tudek² 

[1] Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-474 OLSZTYN, POLAND E-mail:;Genetic Modifications Analysis Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 WARSAW, POLAND E-mail:

关键词: Piezoelectrodes;    Roundup Ready soybean;    Genomic DNA;   

DOI  :  10.3390/s7081462

来源: mdpi

【 摘 要 】

The chemically modified piezoelectrodes were utilized to develop relatively cheap and easy to use biosensor for determination of genetically modified Roundup Ready soybean (RR soybean). The biosensor relies on the immobilization onto gold piezoelectrodes of the 21-mer single stranded oligonucleotide (probes) related to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, which is an active component of an insert integrated into RR soybean genome. The hybridization reaction between the probe and the target complementary sequence in solution was monitored. The system was optimized using synthetic oligonucleotides, which were applied for EPSPS gene detection in DNA samples extracted from animal feed containing 30% RR soybean amplified by the PCR and nonamplified by PCR. The detection limit for genomic DNA was in the range of 4.7·10⁵ numbers of genom copies contained EPSPS gene in the QCM cell. The properties such as sensitivity and selectivity of piezoelectric senor presented here indicated that it could be applied for the direct determination of genetically modified RR soybean in the samples non-amplified by PCR.

【 授权许可】

Unknown   
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