Sensors | |
Piezoelectric Sensor for Determination of Genetically Modified Soybean Roundup Ready® in Samples not Amplified by PCR | |
Magdalena Stobiecka1  Jarosᐪw M. Cieśla2  Beata Janowska2  Barbara Tudek2  | |
[1] Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-474 OLSZTYN, POLAND E-mail:;Genetic Modifications Analysis Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 WARSAW, POLAND E-mail: | |
关键词: Piezoelectrodes; Roundup Ready soybean; Genomic DNA; | |
DOI : 10.3390/s7081462 | |
来源: mdpi | |
【 摘 要 】
The chemically modified piezoelectrodes were utilized to develop relatively cheap and easy to use biosensor for determination of genetically modified Roundup Ready soybean (RR soybean). The biosensor relies on the immobilization onto gold piezoelectrodes of the 21-mer single stranded oligonucleotide (probes) related to 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, which is an active component of an insert integrated into RR soybean genome. The hybridization reaction between the probe and the target complementary sequence in solution was monitored. The system was optimized using synthetic oligonucleotides, which were applied for
【 授权许可】
Unknown
© 2007 by MDPI (
【 预 览 】
Files | Size | Format | View |
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RO202003190058891ZK.pdf | 982KB | download |