Application of Osthol Induces a Resistance Response Against Powdery Mildew in Pumpkin Leaves

International Journal of Molecular Sciences

Zhiqi Shi¹  Fei Wang²  Wei Zhou²  Peng Zhang³ 

[1] Department of Plant Protection of Nanjing Agricultural University, Nanjing210095, China; E-mail:;Food Safety institute, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; E-mail:;Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; E-mail:

关键词: Osthol;    coumarinic compound;    induced resistance response;    Cucurbita moschata;    Sphaerotheca fuliginea;    chitinase;    POD;    PAL;   

DOI  :  10.3390/i8091001

来源: mdpi

【 摘 要 】

Plants can defend themselves against fungal infection by natural means induced by biotic and abiotic elicitors. Osthol is a natural compound extracted from dried fruits of Cnidii Monnieri Fructus. In this study, it has been shown to not only be a fungicide with acceptable curative properties (control efficacy of 68.72), but it also showed a significant prophylactic effect (with control efficacy of 77.36) against pumpkin powdery mildew at a concentration of 100 μg·mL⁻¹. In pumpkin leaves with/or without inoculation of Sphaerotheca fuliginea, osthol treatment induced the accumulation of chitinase and peroxidase and enhanced the transcription of chitinase gene in non-inoculated leaves. The potentiation of phenylalanine amonia-lyase activity in leaves by osthol application and following inoculation was absent in that with inoculation or osthol treatment, indicating that induced PAL in osthol-pretreated plants was inoculation-mediated. In conclusion, this natural compound could induce resistance response in the plant against powdery mildew.

【 授权许可】

Unknown   
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