Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins

Sensors

Nadezhda V. Kulagina¹  George P. Anderson¹  Frances S. Ligler¹  Kara M. Shaffer¹ 

[1] id="af1-sensors-07-02808">Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, U

关键词: Antimicrobial peptide;    botulinum toxin;    detection;    array;    biosensor;   

DOI  :  10.3390/s7112808

来源: mdpi

【 摘 要 】

Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies, differences in binding patterns were observed and can potentially be used for identification of toxoid serotype. Furthermore, some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoid A could be detected at 3.5 LD₅₀ of the active toxin in a 75-min assay, whereas toxoids B and E were detected at 14 and 80 LD₅₀ for their respective toxins.

【 授权许可】

Unknown   
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