Trimethyl Lock: A Stable Chromogenic Substrate for Esterases

Molecules

Michael N. Levine²  Luke D. Lavis¹ 

[1] Department of Chemistry, University of Wisconsin–Madison, 1101 University Avenue, Madison, WI 53706-1322, USA;Department of Biochemistry, University of Wisconsin–Madison, 433 Babcock Drive, Madison, WI 53706-1544, USA

关键词: enzyme catalysis;    chromogenic substrate;    p-nitrophenyl acetate;    trimethyl lock;   

DOI  :  10.3390/molecules13020204

来源: mdpi

【 摘 要 】

p-Nitrophenyl acetate is the most commonly used substrate for detecting the catalytic activity of esterases, including those that activate prodrugs in human cells. This substrate is unstable in aqueous solution, limiting its utility. Here, a stable chromogenic substrate for esterases is produced by the structural isolation of an acetyl ester and p‑nitroaniline group using a trimethyl lock moiety. Upon ester hydrolysis, unfavorable steric interactions between the three methyl groups of this o-hydroxycinnamic acid derivative encourage rapid lactonization to form a hydrocoumarin and release p‑nitroaniline. This “prochromophore” could find use in a variety of assays.

【 授权许可】

Unknown   
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