Redox Behavior of Anticancer Chalcone on a Glassy Carbon Electrode and Evaluation of its Interaction Parameters with DNA

International Journal of Molecular Sciences

Afzal Shah²  Asad M. Khan¹  Rumana Qureshi²  Farzana L. Ansari²  Muhammad F. Nazar² 

[1] id="af1-ijms-09-01424">Deprtment of Chmeistry, Quaid-i-Azam University, 45320 Islamabad, Pakist

关键词: DNA;    hypochromism;    bathochromic shift;    binding constant;   

DOI  :  10.3390/ijms9081424

来源: mdpi

【 摘 要 】

The interaction of anticancer chalcone [AMC, 1-(4′-aminophenyl)-3-(4-N,N-dimethylphenyl)-2-propen-1-one] with DNA has been explored using electrochemical, spectroscopic and viscometric techniques. A shift in peak potential and decrease in peak current were observed in cyclic voltammetry and hypochromism accompanied with bathochromic shift were noticed in UV-Vis absorption spectroscopy. These findings were taken as evidence for AMC –DNA intercalation. A binding constant (K) with a value of 6.15 × 10⁵ M⁻¹ was obtained from CV data, which was also confirmed by UV-Vis absorption titration. Moreover, the diffusion coefficient of the drug with and without DNA (D_b and D_u), heterogeneous electron transfer rate constant (k^o) and electron affinity (A) were also calculated from electrochemical data.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).