Vesicles for Signal Amplification in a Biosensor for the Detection of Low Antigen Concentrations

Sensors

Dorothee Grieshaber²  Victoria de Lange²  Thomas Hirt¹  Zhihua Lu¹ 

[1]BioCure Inc., 2975 Gateway Dr. Suite 100, Norcross, 30071, GA USA E-mails:

[2]ETH Zurich, Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, Gloriastrasse 35, CH-8092 Zurich, Switzerland

关键词: High sensitivity;    antigen detection;    biosensor;    lipid vesicles;    quartz crystal mi-crobalance;   

DOI  :  10.3390/s8127894

来源: mdpi

【 摘 要 】

The sensitivity of biosensors is often not sufficient to detect diagnostically relevant biomarker concentrations. In this paper we have utilized a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) to detect dissipative losses induced by the attachment of intact vesicles. We modified a sandwich assay by coupling the secondary antibodies to vesi-cles. This resulted in an increase of detection sensitivity, achieving a diagnostically relevant detection limit of 5 ng/ml or 30 pM antigens. In addition, we could combine the individual assay steps to decrease the total time to result in about 30 minutes.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.