Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Memórias do Instituto Oswaldo Cruz

Ma.teresa Illnait¹  Juan C Vilaseca¹  Carlos M Fernández¹  Gerardo F Martínez¹ 

[1] ,Instituto Pedro Kourí Laboratorio de Micología Havana,Cuba

关键词: cryptococcal antigen;    antigen quantification;    antigen detection;    enzyme-linked immunosorbent assay-ELISA;   

DOI  :  10.1590/S0074-02762001000200018

来源: SciELO

【 摘 要 】

An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.

【 授权许可】

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