Molecular Weight and Monosaccharide Composition of Astragalus Polysaccharides

Molecules

Du-Juan Xu²  Quan Xia²  Jia-Jia Wang¹ 

[1] School of Pharmacy, Anhui Medical University, Hefei, 230032. P. R. China; E-mails:;Department of Pharmacy, the First Affiliated Hospital of Anhui Medical University, Hefei, 230022. P. R. China; E-mail:

关键词: Astragalus polysaccharides;    Isolation and Purification;    Molecular Weight;    Monosaccharide composition;   

DOI  :  10.3390/molecules13102408

来源: mdpi

【 摘 要 】

Two polysaccharides (APS-I and APS-II) were isolated from the water extract of Radix Astragali and purified through ethanol precipitation, deproteination and by ion-exchange and gel-filtration chromatography. Their molecular weight was determined using high performance liquid chromatography and gel permeation chromatography (HPLC-GPC) and their monosaccharide composition was analyzed by TLC and HPLC methods, using a refractive index detector (RID) and an NH₂ column. It was shown that APS-I consisted of arabinose and glucose and APS-II consisted of rhamnose, arabinose and glucose, in a molar ratio of 1:3.45 and 1:6.25:17.86, respectively. The molecular weights (Mw) of APS-I and APS-II were 1,699,100 Da and 1,197,600 Da, respectively.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.