Binding of Cationic Bis-porphyrins Linked with p- or m- Xylylenediamine and Their Zinc(II) Complexes to Duplex DNA

Molecules

Yoshinobu Ishikawa²  Naoki Yamakawa¹ 

[1]Graduate School of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, Kumamoto 862-0973, Japan

[2] E-mail:

[3]School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan

关键词: Porphyrin;    DNA;    Binding mode;    Zinc;    Titration;    Circular dichroism;    Viscometry;    Distamycin;    Molecular docking simulation.;   

DOI  :  10.3390/molecules13123117

来源: mdpi

【 摘 要 】

Spectroscopic, viscometric, and molecular docking analysis of binding of cationic bis-porphyrins linked with p- or m-xylylenediamine (H₂pXy and H₂mXy) and their zinc(II) complexes (ZnpXy and ZnmXy) to duplex DNA are described. H₂pXy and H₂mXy bound to calf thymus DNA (CTDNA) stronger than unichromophoric H₂TMPyP, and showed exciton-type induced circular dichroism spectra of their Soret bands. The H₂TMPyP-like units of the metal-free bis-porphyrins did not intercalate into CTDNA, and thus the binding mode is outside binding with intramolecular stacking. ZnpXy showed favorable binding to A·T over G·C region, and should lie in the major groove of A·T region.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.