Potential Use of Quantum Dots in Flow Cytometry

International Journal of Molecular Sciences

Raquel Ibá༞z-Peral³  Peter L. Bergquist³  Malcolm R. Walter²  Moreland Gibbs³  Ewa M. Goldys¹ 

[1] Department of Physics, Macquarie University, Sydney, Australia. E-Mail:;Department of Earth Sciences, Macquarie University, Sydney, Australia. E-Mail:;Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia. E-Mails:

关键词: Quantum dots;    flow cytometry;    fluorescent detection;    detection limit;    autofluorescence;   

DOI  :  10.3390/ijms9122622

来源: mdpi

【 摘 要 】

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.

【 授权许可】

CC BY   
© 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).