Molecularly Imprinted Electrochemical Sensor-Based Fe2O3@MWCNTs for Ivabradine Drug Determination in Pharmaceutical Formulation, Serum, and Urine Samples

Frontiers in Bioengineering and Biotechnology

Fatehy M. Abdel-Haleem¹  Adel Madbouly²  Rasha M. El Nashar²  Eman Gamal²  Mahmoud S. Rizk²  Hussam M. Elnabawy³  Ahmed S. G. Khalil⁴  Ahmed Barhoum⁶  Badawi Anis⁷ 

[1] Cairo University Centre for Environmental Hazards Mitigation, Environmental Studies and Research, CHMESR, Cairo University, Giza, Egypt;Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt;Environmental and Smart Technology Group (ESTG), Faculty of Science, Fayoum University, Faiyum, Egypt;Materials Science and Engineering Department, School of Innovative Design Engineering, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City, Egypt;NanoStruc. Research Group, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt;National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin, Ireland;Spectroscopy Department, National Research Centre, Giza, Egypt;

关键词: carbon paste electrodes;    molecularly imprinted polymers;    detection limit;    formation constant;    selectivity against interfering species;    H-bonding complexing strength;   

DOI  :  10.3389/fbioe.2021.648704

来源: DOAJ

【 摘 要 】

Ivabradine hydrochloride (IVR) is a medically important drug because of its ability to lower the heart rate. Techniques reported for IVR determination were expensive, laborious, besides being of poor selectivity. In this study, iron oxide @ carbon nanotube (Fe2O3@MWCNTs) nanocomposite and molecularly imprinted polymer (MIP) were synthesized and used in the fabrication of carbon paste electrodes (CPEs) for the potentiometric detection of IVR in biological and pharmaceutical samples. CPEs of the best sensor were formulated from graphite (41 wt%) as a carbon source, MIP (3 wt.%) as an ionophore, Fe2O3@MWCNTs (5 wt%) as a modifier, and nitrophenyl octyl ether (NPOE, 51 wt.%) as a conductive oil so-called plasticizer. The best sensor exhibits a Nernstian slope (response) of 56 mV decade–1 within the IVR concentration range from 1.0 × 10–3 M to 9.8 × 10–8 M with high selectivity against interfering species (ascorbic, maltose, glucose, lactose, dopamine, glycine) over those reported earlier. The use of Fe2O3@MWCNTs together with MIP in the electrode formulation was found to improve the limit of detection (LOD) from 630 to 98 nM along with high reversibility, a short response time of 30 s, and a good lifetime of more than 2 weeks. The sandwich membrane (SMM) method was used to quantify the H-bonding complexing strength of the MIP binding sites for IVR with Log βILn = 11.33. The constructed sensors were successfully applied for the IVR determination in blood serum, urine, and commercial formulations (Savapran®) with high sensitivity.

【 授权许可】

Unknown