Metabolic Discrimination of Select List Agents by Monitoring Cellular Responses in a Multianalyte Microphysiometer

Sensors

Sven E. Eklund¹  Roy G. Thompson³  Rachel M. Snider¹  Clare K. Carney¹  David W. Wright¹  John Wikswo² 

[1] Department of Chemistry, Vanderbilt University, VU Station B. Nashville, TN 37235, USA; E-Mails:;Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, VU Station B. Nashville, TN 37235. USA; E-Mail:;Edgewood Chemical and Biological Center, Aberdeen Proving Ground, MD 21010. USA; E-Mail:

关键词: Toxin;    biotoxin;    biosensors;    microphysiometry;   

DOI  :  10.3390/s90302117

来源: mdpi

【 摘 要 】

Harnessing the potential of cells as complex biosensors promises the potential to create sensitive and selective detectors for discrimination of biodefense agents. Here we present toxin detection and suggest discrimination using cells in a multianalyte microphysiometer (MMP) that is capable of simultaneously measuring flux changes in four extracellular analytes (acidification rate, glucose uptake, oxygen uptake, and lactate production) in real-time. Differential short-term cellular responses were observed between botulinum neurotoxin A and ricin toxin with neuroblastoma cells, alamethicin and anthrax protective antigen with RAW macrophages, and cholera toxin, muscarine, 2,4-dinitro-phenol, and NaF with CHO cells. These results and the post exposure dynamics and metabolic recovery observed in each case suggest the usefulness of cell-based detectors to discriminate between specific analytes and classes of compounds in a complex matrix, and furthermore to make metabolic inferences on the cellular effects of the agents. This may be particularly valuable for classifying unknown toxins.

【 授权许可】

CC BY   
© 2009 by the authors; licensee MDPI, Basel, Switzerland