Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays

International Journal of Molecular Sciences

Gerard Giraud⁵  Holger Schulze³  Till T. Bachmann³  Colin J. Campbell³  Andrew R.²  rib-type="author">Mount34  Peter Ghazal³  Mizanur R. Khondoker³  Alan J. Ross³  Stuart W. J. Ember³  Ilenia Ciani⁴  Chaker Tlili⁴  Anthony J. Walton¹  Jonathan G. Terry¹ 

[1] Institute of Integrated Systems, School of Engineering, The University of Edinburgh, The King’s Buildings, West Mains Road, Edinburgh EH9 3JF, Scotland, UK; E-Mails:;School of Physics and Astronomy, The University of Edinburgh, The King’s Buildings, West Mains Road, Edinburgh EH9 3JZ, Scotland, UK; E-Mai;Division of Pathway Medicine, Medical School, The University of Edinburgh, Chancellor’s Building, Little France Crescent, Edinburgh EH16 4SB, Scotland, UK; E-Mails:;School of Chemistry, The University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, EH9 3JJ, Scotland, UK; E-Mails:;School of Physics and Astronomy, The University of Edinburgh, The King’s Buildings, West Mains Road, Edinburgh EH9 3JZ, Scotland, UK; E-Mail:

关键词: Quantum Dots (QD);    Fluorescence Lifetime Imaging Microscopy (FLIM);    DNA Microarray;    Lifetime Multiplexing;   

DOI  :  10.3390/ijms10041930

来源: mdpi

【 摘 要 】

Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.