Production of DNA microarray and expression analysis of genes from Xylella fastidiosa in different culture media

Brazilian Archives of Biology and Technology

Regiane De Fátima Travensolo²  Maria Vitória Cecchette Gottardi Costa²  Lucia Maria Carareto-alves²  Emanuel Carrilho¹  Eliana Gertrudes De Macedo Lemos² 

[1] ,Universidade Estadual Paulista Departamento de Tecnologia Jaboticabal SP ,Brasil

关键词: DNA Microarray;    Xylella fastidiosa;    expression;    transcriptome;   

DOI  :  10.1590/S1516-89132009000300006

来源: SciELO

【 摘 要 】

DNA Microarray was developed to monitor the expression of many genes from Xylella fastidiosa, allowing the side by-side comparison of two situations in a single experiment. The experiments were performed using X. fastidiosa cells grown in two culture media: BCYE and XDM2. The primers were synthesized, spotted onto glass slides and the array was hybridized against fluorescently labeled cDNAs. The emitted signals were quantified, normalized and the data were statistically analyzed to verify the differentially expressed genes. According to the data, 104 genes were differentially expressed in XDM2 and 30 genes in BCYE media. The present study showed that DNA microarray technique efficiently differentiate the expressed genes under different conditions.

【 授权许可】

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