Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems

Sensors

Clarissa Lui²  Nathaniel C. Cady¹ 

[1] College of Nanoscale Science and Engineering / University at Albany State University of New York, 255 Fuller Rd., Albany, NY 12203, USA; E-Mail:;Department of Biomedical Engineering / Cornell University, 317 Stocking Hall, Ithaca, NY 14853, USA

关键词: pathogen;    sensors;    biosensors;    PCR;    DNA;    microfluidics;    μTAS;    lab-on-a-chip;   

DOI  :  10.3390/s90503713

来源: mdpi

【 摘 要 】

The advent of nucleic acid-based pathogen detection methods offers increased sensitivity and specificity over traditional microbiological techniques, driving the development of portable, integrated biosensors. The miniaturization and automation of integrated detection systems presents a significant advantage for rapid, portable field-based testing. In this review, we highlight current developments and directions in nucleic acid-based micro total analysis systems for the detection of bacterial pathogens. Recent progress in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and purification, polymerase chain reaction, and product detection are detailed. Discussions include strategies and challenges for implementation of an integrated portable platform.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.