Microscopic Investigation of Reversible Nanoscale Surface Size Dependent Protein Conjugation

International Journal of Molecular Sciences

Kazushige Yokoyama²  Hyunah Cho²  Sean P. Cullen²  Matthew Kowalik²  Nicole M. Briglio²  Harold J. Hoops¹  Zhouying Zhao³ 

[1] Department of Biology, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA; E-Mail:;Department of Chemistry, The State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA; E-Mails:;College of Nanoscale Science and Engineering, University of Albany-SUNY, 255 Fuller Road, Albany, NY 12203, USA; E-Mails:

关键词: amyloid beta;    fibrillogenesis;    Alzheimer’s disease;    ovalbumin;    gold nanoparticles;    AFM;    TEM;   

DOI  :  10.3390/ijms10052348

来源: mdpi

【 摘 要 】

Aβ_1–40 coated 20 nm gold colloidal nanoparticles exhibit a reversible color change as pH is externally altered between pH 4 and 10. This reversible process may contain important information on the initial reversible step reported for the fibrillogenesis of Aβ (a hallmark of Alzheimer’s disease). We examined this reversible color change by microscopic investigations. AFM images on graphite surfaces revealed the morphology of Aβ aggregates with gold colloids. TEM images clearly demonstrate the correspondence between spectroscopic features and conformational changes of the gold colloid.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.