Effect of Potato Virus Y on the NADP-Malic Enzyme from Nicotiana tabacum L.: mRNA, Expressed Protein and Activity

International Journal of Molecular Sciences

Veronika Doubnerová³  Karel Müller¹  Noemi ჎řovská²  Helena Synková²  Petra Spoustová² 

[1] Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, Prague 6, 165 02, Czech Republic; E-Mail:;Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1a, Prague 6, 160 00, Czech Republic; E-Mails:;Department of Biochemistry, Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czech Republic; E-Mail:

关键词: NADP-malic enzyme;    Nicotiana tabacum L.;    Potato virus Y strain NTN (PVY^NTN);    Potato virus Y strain O (PVY^O);    biotic stress;    real time-PCR;   

DOI  :  10.3390/ijms10083583

来源: mdpi

【 摘 要 】

The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic NADP-ME isoform. Transcription of the chloroplastic enzyme was not influenced by viral infection. The increase of the enzyme activity was also detected in stems and roots of infected plants. The effect of viral infection induced by Potato virus Y, NTN strain, causing more severe symptoms, was compared with the effect induced by milder strain PVY^O. The observed increase in NADP-malic enzyme activity in all parts of the studied plants was higher in the case of PVY^NTN strain than in the case of strain PVY^O. The relevance of NADP-malic enzyme in plants under stress conditions was discussed.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.