Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings

International Journal of Molecular Sciences

Maria Dimou¹  Anca Paunescu²  Georgios Aivalakis¹  Emmanouil Flemetakis¹ 

[1]Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Botanikos, Athens, Greece

[2] E-Mails:

[3]Institute of Biology, Splaiul Independentei 296, Bucharest 060031, Romania

[4] E-Mail:

关键词: carbonic anhydrase;    Glycine max;    hypocotyl;    in situ hybridization;    phosphoenolpyruvate carboxylase;    pyruvate kinase;    root;   

DOI  :  10.3390/ijms10072896

来源: mdpi

【 摘 要 】

We investigated the presence of carbonic anhydrase in root and hypocotyl of etiolated soybean using enzymatic, histochemical, immunohistochemical and in situ hybridization approaches. In parallel, we used in situ hybridization and immunolocalization to determine the expression pattern and localization of phosphoenolpyruvate carboxylase. Their co-localization in the root tip as well as in the central cylinder, suggests that a large fraction of the CO₂ may be re-introduced into C4 compounds. GmPK3 expression, coding for a cytoplasmic isoform of pyruvate kinase, was detected in all different root cell types, suggesting that both phosphoenolpyruvate-utilizing enzymes are involved in phosphoenolpyruvate metabolism in etiolated soybean roots; a case indicative of the necessary flexibility plant metabolism has to adopt in order to compensate various physiological conditions.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.