Antioxidant and Cytotoxic Activities of Centella asiatica (L) Urb.

International Journal of Molecular Sciences

Frederico Pittella¹  Rafael C. Dutra¹  Dalton D. Junior²  Miriam T. P. Lopes² 

[1] Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brazil; E-Mail:;Laboratório de Substâncias Antitumorais, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil; E-Mail:

关键词: Centella asiatica;    Umbelliferae;    phenolic constituents;    DPPH;    cytotoxic;   

DOI  :  10.3390/ijms10093713

来源: mdpi

【 摘 要 】

In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC₅₀ value of 31.25 μg/mL. The AE had a promising activity against mouse melanoma (B₁₆F₁), human breast cancer (MDA MB-231) and rat glioma (C₆) cell lines, with IC₅₀ values of 698.0, 648.0 and 1000.0 μg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.